Capillary electrophoresis as a versatile tool for quality control and epidermis permeation studies of transdermal formulations

Capillary electrophoresis has been investigated to evaluate the performances of new transdermal formulations containing antiemetics. After optimization background electrolyte (sodium phosphate buffer (pH 2.5; 60 mM) 12% ethanol (v/v)), domperidone, diphenhydramine, haloperidol, metoclopramide and promethazine were base-line resolved in 10 min. hydrodynamic injection sample (0.5 psi for 7 s), method was fully validated through build accuracy profile. Trueness values ranged from ?1.85 5.43% relative standard deviation intra-day inter-day precision lower than 6.20%. This found convenient quality control extemporaneous ready-to-use with recoveries ranging 91.2–107.8%. However, using injection, limits quantitation 0.3–2.6 ?g.mL?1 range, not low enough permeation rate antimetics epidermis. Field amplified used improve both sensitivity thresholds. Several parameters (nature concentration protonation agent, composition injected solvent, applied voltage duration injection) have optimized a multivariate approach. In conditions, signal-to-noise ratios improved by 600- 2000-fold factor, regarding antiemetic. presence salts simulated body fluid solution, as receptor medium perform kinetic study, improper allow stacking effect. Therefore, liquid-liquid extraction developed on solution. Finally, this shown strongly useful pig